Detailed information of TA system TA093700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3028969..3029519
• Replicon: chromosome
• Accession: NZ_CP026216
• Organism: Citrobacter sp. CFNIH10

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: C2U53_RS18065
• Protein ID: WP_007372286.1
• Coordinates: 3028969..3029277 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: C2U53_RS18070
• Protein ID: WP_007372285.1
• Coordinates: 3029280..3029519 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U53_RS18025	3024423..3025361	+	939	WP_000449980.1	MCE family protein	-
C2U53_RS18030	3025361..3026002	+	642	WP_000948259.1	membrane integrity-associated transporter subunit PqiC	-
C2U53_RS18040	3026122..3026819	+	698	Protein_2767	IS1 family transposase	-
C2U53_RS18045	3026829..3027167	+	339	Protein_2768	murein transglycosylase B	-
C2U53_RS18050	3027231..3027935	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C2U53_RS18060	3028081..3028635	-	555	WP_007372288.1	hypothetical protein	-
C2U53_RS29955	3028790..3028948	-	159	WP_016241558.1	hypothetical protein	-
C2U53_RS18065	3028969..3029277	-	309	WP_007372286.1	CcdB family protein	Toxin
C2U53_RS18070	3029280..3029519	-	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C2U53_RS30105	3029658..3030122	-	465	WP_008786596.1	hypothetical protein	-
C2U53_RS18080	3030119..3030790	-	672	WP_007372283.1	hypothetical protein	-
C2U53_RS18085	3031251..3031883	-	633	WP_007372282.1	hypothetical protein	-
C2U53_RS29960	3031899..3032039	-	141	WP_007372281.1	hypothetical protein	-
C2U53_RS18090	3032187..3032645	-	459	WP_035942286.1	hypothetical protein	-
C2U53_RS18095	3032698..3032895	-	198	WP_032155220.1	Lar family restriction alleviation protein	-
C2U53_RS18100	3032918..3033613	-	696	WP_007372278.1	hypothetical protein	-
C2U53_RS18105	3033744..3033935	-	192	WP_016241560.1	hypothetical protein	-
C2U53_RS18110	3034117..3034512	-	396	WP_024196081.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T93700 WP_007372286.1 NZ_CP026216:c3029277-3028969 [Citrobacter sp. CFNIH10]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T93700 NZ_CP026216:c3029277-3028969 [Citrobacter sp. CFNIH10]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT93700 WP_007372285.1 NZ_CP026216:c3029519-3029280 [Citrobacter sp. CFNIH10]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT93700 NZ_CP026216:c3029519-3029280 [Citrobacter sp. CFNIH10]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3