Detailed information of TA system TA093697

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2054310..2055031
• Replicon: chromosome
• Accession: NZ_CP026216
• Organism: Citrobacter sp. CFNIH10

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: C2U53_RS13375
• Protein ID: WP_104446003.1
• Coordinates: 2054690..2055031 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C2U53_RS13370
• Protein ID: WP_104446002.1
• Coordinates: 2054310..2054654 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U53_RS13335	2050676..2051110	+	435	WP_104445997.1	hypothetical protein	-
C2U53_RS13340	2051130..2051933	+	804	WP_104445998.1	hypothetical protein	-
C2U53_RS13350	2052165..2052620	+	456	WP_104445999.1	hypothetical protein	-
C2U53_RS13355	2052689..2053510	+	822	WP_104446000.1	DUF945 domain-containing protein	-
C2U53_RS13360	2053581..2054054	+	474	WP_047399355.1	DNA repair protein RadC	-
C2U53_RS13365	2054065..2054286	+	222	WP_104446001.1	DUF987 family protein	-
C2U53_RS13370	2054310..2054654	+	345	WP_104446002.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C2U53_RS13375	2054690..2055031	+	342	WP_104446003.1	TA system toxin CbtA family protein	Toxin
C2U53_RS13380	2055146..2055979	+	834	WP_032676675.1	DUF4942 domain-containing protein	-
C2U53_RS13385	2056056..2056304	+	249	WP_000535212.1	ribbon-helix-helix domain-containing protein	-
C2U53_RS13390	2056308..2056583	+	276	WP_104446004.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U53_RS13395	2056698..2057495	+	798	WP_104446005.1	helix-turn-helix transcriptional regulator	-
C2U53_RS13400	2057784..2058530	-	747	WP_061069582.1	epimerase	-
C2U53_RS13405	2058541..2058831	-	291	WP_044264508.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
C2U53_RS13410	2058880..2059755	-	876	WP_000774153.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2034122..2057495	23373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.83 Da
• Isoelectric Point: 9.0089

>T93697 WP_104446003.1 NZ_CP026216:2054690-2055031 [Citrobacter sp. CFNIH10]
MKTFPATTQRAAKPCPSPVAVWQMLLARLLEQHYGLTLSDTPFNDESIIQEHINAGITLADAVNFLVEKYELVRIDRRGF
SWQEQSPYLRAVDILRARQAMGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T93697 NZ_CP026216:2054690-2055031 [Citrobacter sp. CFNIH10]
ATGAAAACCTTTCCTGCAACAACTCAGCGGGCGGCGAAGCCATGCCCGTCGCCTGTGGCCGTCTGGCAGATGTTACTTGC
CCGTCTACTTGAGCAGCACTATGGCCTGACCCTTAGCGATACTCCATTCAATGATGAAAGTATTATTCAGGAACATATCA
ACGCCGGTATCACACTAGCCGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTCGTGCGCATCGATCGCAGGGGATTT
AGCTGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGACATTTTGCGGGCGCGACAGGCAATGGGGTTGTTACGACAAGG
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12989.02 Da
• Isoelectric Point: 8.1539

>AT93697 WP_104446002.1 NZ_CP026216:2054310-2054654 [Citrobacter sp. CFNIH10]
MKKDTANEVVDTLRWGLKCDITPHFGARLVQEGNRLHYLADRASIIGQFSKADLRHLNQAFPLLMKQMELMLTSGELNPR
HQHCITLYAKRLTCEADTLGSCGYVYVAIYPTQH

Nucleotide

• Length: 345 bp

>AT93697 NZ_CP026216:2054310-2054654 [Citrobacter sp. CFNIH10]
ATGAAAAAAGACACAGCCAATGAAGTAGTGGATACGCTGCGATGGGGACTGAAATGCGACATCACACCACATTTCGGGGC
CCGTCTGGTGCAGGAAGGCAACCGCCTGCATTACCTCGCCGATCGGGCCAGCATAATTGGTCAGTTCAGCAAAGCTGATT
TACGCCATCTCAACCAGGCTTTCCCCTTGTTGATGAAGCAAATGGAGCTCATGCTCACCAGCGGAGAACTTAATCCCCGG
CACCAGCACTGCATCACGTTGTATGCGAAAAGATTAACCTGTGAAGCCGATACGCTTGGTTCTTGTGGCTACGTCTATGT
CGCCATTTACCCCACTCAACACTGA