Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 567352..568031 Replicon chromosome
Accession NZ_CP026216
Organism Citrobacter sp. CFNIH10

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1Z3N0J5
Locus tag C2U53_RS06010 Protein ID WP_045268641.1
Coordinates 567690..568031 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A1Z3N0L6
Locus tag C2U53_RS06005 Protein ID WP_045268640.1
Coordinates 567352..567669 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U53_RS05980 562672..563826 - 1155 WP_045268636.1 UvrD-helicase domain-containing protein -
C2U53_RS05985 563819..565477 - 1659 WP_045268637.1 AAA family ATPase -
C2U53_RS29865 565866..566050 + 185 Protein_516 DUF905 family protein -
C2U53_RS05990 566152..566610 + 459 WP_045268638.1 antirestriction protein -
C2U53_RS05995 566626..567102 + 477 WP_045268639.1 RadC family protein -
C2U53_RS06000 567111..567332 + 222 WP_007893437.1 DUF987 domain-containing protein -
C2U53_RS06005 567352..567669 + 318 WP_045268640.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C2U53_RS06010 567690..568031 + 342 WP_045268641.1 TA system toxin CbtA family protein Toxin
C2U53_RS06020 568394..568900 + 507 WP_044265577.1 G/U mismatch-specific DNA glycosylase -
C2U53_RS06025 568948..570792 - 1845 WP_042998197.1 RNA polymerase sigma factor RpoD -
C2U53_RS06030 571026..572771 - 1746 WP_044265580.1 DNA primase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mrkA / mrkB / mrkC / mrkD / mrkF 535630..568239 32609


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13045.30 Da        Isoelectric Point: 10.5786

>T93693 WP_045268641.1 NZ_CP026216:567690-568031 [Citrobacter sp. CFNIH10]
MKTLPATTLRVVKPCLSPVAIWKMLLTRLLEQHYGLTLNDTPFSKERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Download         Length: 342 bp

>T93693 NZ_CP026216:567690-568031 [Citrobacter sp. CFNIH10]
ATGAAAACTTTACCTGCAACAACTCTGCGGGTGGTGAAGCCCTGCCTGTCACCCGTGGCTATCTGGAAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTAAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGGTTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11976.67 Da        Isoelectric Point: 6.7379

>AT93693 WP_045268640.1 NZ_CP026216:567352-567669 [Citrobacter sp. CFNIH10]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGMFSDVECLKLDDTFQHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT93693 NZ_CP026216:567352-567669 [Citrobacter sp. CFNIH10]
ATGACCCACATCATCTGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTACAGGAGGGCAACCGACT
GCACTACCTGGCTGACCGGGCCAGCATCACCGGTATGTTCAGTGACGTCGAATGCCTGAAGCTGGATGACACATTCCAGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCAACATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3N0J5

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3N0L6

References