Detailed information of TA system TA093691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 136786..137687
• Replicon: plasmid pKPC-933d
• Accession: NZ_CP026213
• Organism: Citrobacter sp. CFNIH10

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: C2U53_RS02535
• Protein ID: WP_007372300.1
• Coordinates: 137229..137687 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: C2U53_RS02530
• Protein ID: WP_008786588.1
• Coordinates: 136786..137232 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U53_RS02500	132149..132400	-	252	WP_024196080.1	hypothetical protein	-
C2U53_RS02505	132822..133664	-	843	WP_007372307.1	HNH endonuclease	-
C2U53_RS29800	134232..134396	-	165	WP_007372306.1	hypothetical protein	-
C2U53_RS02510	134683..135126	-	444	WP_007372305.1	hypothetical protein	-
C2U53_RS02515	135186..135464	-	279	WP_007372304.1	hypothetical protein	-
C2U53_RS02520	135436..136278	-	843	WP_007372303.1	hypothetical protein	-
C2U53_RS02525	136320..136637	-	318	WP_007372302.1	hypothetical protein	-
C2U53_RS02530	136786..137232	+	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
C2U53_RS02535	137229..137687	+	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
C2U53_RS02540	137731..137961	+	231	WP_007372299.1	hypothetical protein	-
C2U53_RS02545	138029..138295	-	267	WP_007372298.1	hypothetical protein	-
C2U53_RS02550	138345..138899	-	555	WP_007372297.1	hypothetical protein	-
C2U53_RS02555	139058..139399	-	342	WP_007372296.1	hypothetical protein	-
C2U53_RS02560	139518..139961	-	444	WP_007372295.1	hypothetical protein	-
C2U53_RS02565	140199..140537	+	339	WP_007372294.1	hypothetical protein	-
C2U53_RS02570	140900..141226	-	327	WP_007372293.1	hypothetical protein	-
C2U53_RS02575	141310..141708	-	399	WP_007372292.1	hypothetical protein	-
C2U53_RS02580	141719..141961	-	243	WP_007372291.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / blaKPC-2 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..221962	221962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T93691 WP_007372300.1 NZ_CP026213:137229-137687 [Citrobacter sp. CFNIH10]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T93691 NZ_CP026213:137229-137687 [Citrobacter sp. CFNIH10]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT93691 WP_008786588.1 NZ_CP026213:136786-137232 [Citrobacter sp. CFNIH10]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT93691 NZ_CP026213:136786-137232 [Citrobacter sp. CFNIH10]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A837LJP8