Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 69881..70524 Replicon plasmid pCIT-a850
Accession NZ_CP026212
Organism Citrobacter sp. CFNIH10

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2U53_RS01350 Protein ID WP_001044770.1
Coordinates 70108..70524 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2U53_RS01345 Protein ID WP_001261282.1
Coordinates 69881..70111 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U53_RS01315 66003..66638 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
C2U53_RS01320 66746..67087 - 342 WP_071844672.1 hypothetical protein -
C2U53_RS01325 67410..68346 + 937 Protein_86 CAAX protease -
C2U53_RS01330 68537..68977 - 441 WP_047057603.1 hypothetical protein -
C2U53_RS01335 68996..69589 - 594 WP_009309909.1 hypothetical protein -
C2U53_RS01340 69688..69924 - 237 Protein_89 hypothetical protein -
C2U53_RS01345 69881..70111 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2U53_RS01350 70108..70524 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2U53_RS01355 70598..72157 + 1560 WP_103215780.1 AAA family ATPase -
C2U53_RS01360 72199..73167 - 969 WP_072269234.1 IS5 family transposase -
C2U53_RS01365 73211..74233 + 1023 WP_000361404.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..126940 126940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93689 WP_001044770.1 NZ_CP026212:70108-70524 [Citrobacter sp. CFNIH10]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93689 NZ_CP026212:70108-70524 [Citrobacter sp. CFNIH10]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93689 WP_001261282.1 NZ_CP026212:69881-70111 [Citrobacter sp. CFNIH10]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93689 NZ_CP026212:69881-70111 [Citrobacter sp. CFNIH10]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References