Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60404..61047 Replicon plasmid pCIT-a850
Accession NZ_CP026212
Organism Citrobacter sp. CFNIH10

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag C2U53_RS01295 Protein ID WP_020804312.1
Coordinates 60631..61047 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag C2U53_RS01290 Protein ID WP_001261276.1
Coordinates 60404..60634 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U53_RS30085 55552..55854 + 303 WP_071571079.1 hypothetical protein -
C2U53_RS01250 56358..57152 - 795 WP_004197635.1 site-specific integrase -
C2U53_RS01255 57350..58366 - 1017 WP_017899884.1 hypothetical protein -
C2U53_RS01260 58377..58691 - 315 WP_053389906.1 hypothetical protein -
C2U53_RS01265 58718..59113 - 396 WP_017899885.1 hypothetical protein -
C2U53_RS01270 59282..59587 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
C2U53_RS01275 59589..59807 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
C2U53_RS01290 60404..60634 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2U53_RS01295 60631..61047 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
C2U53_RS01300 61088..61966 - 879 WP_032427228.1 restriction endonuclease -
C2U53_RS01305 62628..63905 + 1278 WP_103214963.1 HlyD family secretion protein -
C2U53_RS01310 63895..66003 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..126940 126940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T93688 WP_020804312.1 NZ_CP026212:60631-61047 [Citrobacter sp. CFNIH10]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T93688 NZ_CP026212:60631-61047 [Citrobacter sp. CFNIH10]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT93688 WP_001261276.1 NZ_CP026212:60404-60634 [Citrobacter sp. CFNIH10]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT93688 NZ_CP026212:60404-60634 [Citrobacter sp. CFNIH10]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References