Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59282..59807 Replicon plasmid pCIT-a850
Accession NZ_CP026212
Organism Citrobacter sp. CFNIH10

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag C2U53_RS01270 Protein ID WP_013023785.1
Coordinates 59282..59587 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag C2U53_RS01275 Protein ID WP_001568025.1
Coordinates 59589..59807 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U53_RS01240 54929..55555 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
C2U53_RS30085 55552..55854 + 303 WP_071571079.1 hypothetical protein -
C2U53_RS01250 56358..57152 - 795 WP_004197635.1 site-specific integrase -
C2U53_RS01255 57350..58366 - 1017 WP_017899884.1 hypothetical protein -
C2U53_RS01260 58377..58691 - 315 WP_053389906.1 hypothetical protein -
C2U53_RS01265 58718..59113 - 396 WP_017899885.1 hypothetical protein -
C2U53_RS01270 59282..59587 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
C2U53_RS01275 59589..59807 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C2U53_RS01290 60404..60634 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C2U53_RS01295 60631..61047 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
C2U53_RS01300 61088..61966 - 879 WP_032427228.1 restriction endonuclease -
C2U53_RS01305 62628..63905 + 1278 WP_103214963.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..126940 126940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T93687 WP_013023785.1 NZ_CP026212:c59587-59282 [Citrobacter sp. CFNIH10]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T93687 NZ_CP026212:c59587-59282 [Citrobacter sp. CFNIH10]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT93687 WP_001568025.1 NZ_CP026212:c59807-59589 [Citrobacter sp. CFNIH10]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT93687 NZ_CP026212:c59807-59589 [Citrobacter sp. CFNIH10]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References