Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 12791..13338 Replicon plasmid pECO-6dfa
Accession NZ_CP026200
Organism Escherichia coli strain ECONIH6

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag C2U45_RS25170 Protein ID WP_000969996.1
Coordinates 12791..13072 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag C2U45_RS25175 Protein ID WP_021546940.1
Coordinates 13069..13338 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U45_RS25145 8780..9664 - 885 WP_000058717.1 EamA family transporter -
C2U45_RS25150 9802..10209 - 408 WP_001214976.1 cysteine hydrolase -
C2U45_RS25155 10198..11949 + 1752 Protein_12 Tn3-like element TnAs1 family transposase -
C2U45_RS25160 12250..12459 - 210 Protein_13 transposase -
C2U45_RS26725 12533..12745 - 213 Protein_14 3'-5' exonuclease -
C2U45_RS25170 12791..13072 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C2U45_RS25175 13069..13338 - 270 WP_021546940.1 hypothetical protein Antitoxin
C2U45_RS25185 14085..14696 + 612 Protein_17 IS3 family transposase -
C2U45_RS25190 14728..14907 - 180 WP_012600012.1 hypothetical protein -
C2U45_RS25195 15131..15448 + 318 WP_032156934.1 hypothetical protein -
C2U45_RS25200 15517..16443 + 927 WP_000617209.1 WYL domain-containing protein -
C2U45_RS25205 16453..17052 + 600 WP_001021938.1 DUF1819 family protein -
C2U45_RS25210 17049..17633 + 585 WP_000645940.1 DUF1788 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / tet(A) / qnrS1 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..97800 97800
- inside IScluster/Tn dfrA14 / tet(A) - 422..14696 14274


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T93682 WP_000969996.1 NZ_CP026200:c13072-12791 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T93682 NZ_CP026200:c13072-12791 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCATCCCCATTTTGTTCTGGTTTATGAAGTCGATAGCCAGTGGGGTAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT93682 WP_021546940.1 NZ_CP026200:c13338-13069 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT93682 NZ_CP026200:c13338-13069 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGTGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGCGCAGGAAGGTTGCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References