Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 250198..250724 Replicon plasmid pKPN-3967
Accession NZ_CP026186
Organism Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C2U49_RS32935 Protein ID WP_000323025.1
Coordinates 250437..250724 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag C2U49_RS32930 Protein ID WP_004196370.1
Coordinates 250198..250437 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U49_RS32900 245535..245727 - 193 Protein_256 MFS transporter -
C2U49_RS32905 245757..246734 + 978 WP_004196334.1 chromate resistance protein -
C2U49_RS32910 246691..248067 + 1377 WP_004196363.1 chromate efflux transporter -
C2U49_RS32915 248098..248787 - 690 WP_004196322.1 hypothetical protein -
C2U49_RS32920 248801..249538 - 738 WP_008460272.1 HupE/UreJ family protein -
C2U49_RS32925 249582..249947 - 366 WP_009651956.1 hypothetical protein -
C2U49_RS32930 250198..250437 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C2U49_RS32935 250437..250724 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C2U49_RS32940 250796..250954 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
C2U49_RS34545 251560..252534 + 975 WP_004196336.1 hypothetical protein -
C2U49_RS32950 252732..253109 - 378 WP_004196351.1 hypothetical protein -
C2U49_RS32965 253799..254866 + 1068 WP_004181903.1 hypothetical protein -
C2U49_RS32970 255022..255369 + 348 WP_004196344.1 hypothetical protein -
C2U49_RS32975 255448..255681 + 234 WP_004196327.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..373179 373179


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T93681 WP_000323025.1 NZ_CP026186:250437-250724 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T93681 NZ_CP026186:250437-250724 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT93681 WP_004196370.1 NZ_CP026186:250198-250437 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT93681 NZ_CP026186:250198-250437 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References