Detailed information of TA system TA093680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 233243..234153
• Replicon: plasmid pKPN-3967
• Accession: NZ_CP026186
• Organism: Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2N4Z2R0
• Locus tag: C2U49_RS32835
• Protein ID: WP_004197882.1
• Coordinates: 233683..234153 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: C2U49_RS32830
• Protein ID: WP_004181895.1
• Coordinates: 233243..233686 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U49_RS32805	229185..229991	-	807	WP_048268727.1	SagB/ThcOx family dehydrogenase	-
C2U49_RS32810	229993..230847	-	855	WP_048268726.1	McbB family protein	-
C2U49_RS32815	230906..231115	-	210	WP_072072310.1	microcin B17	-
C2U49_RS32820	231366..231824	-	459	Protein_241	recombinase family protein	-
C2U49_RS32825	231967..233142	+	1176	Protein_242	translesion error-prone DNA polymerase V subunit UmuC	-
C2U49_RS32830	233243..233686	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
C2U49_RS32835	233683..234153	+	471	WP_004197882.1	RES family NAD+ phosphorylase	Toxin
C2U49_RS32840	234263..234523	-	261	WP_004026352.1	hypothetical protein	-
C2U49_RS32845	235085..236266	-	1182	WP_061891937.1	IS481 family transposase	-
C2U49_RS32850	236407..237939	+	1533	WP_040088343.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..373179	373179
	-	inside	IScluster/Tn	-	-	231366..237939	6573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17633.92 Da
• Isoelectric Point: 4.5152

>T93680 WP_004197882.1 NZ_CP026186:233683-234153 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T93680 NZ_CP026186:233683-234153 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT93680 WP_004181895.1 NZ_CP026186:233243-233686 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT93680 NZ_CP026186:233243-233686 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2N4Z2R0

Antitoxin

• AlphaFold DB: A0A2J4RLY9