Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35486..36011 Replicon plasmid pKPN-9a0d
Accession NZ_CP026185
Organism Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag C2U49_RS31450 Protein ID WP_013023785.1
Coordinates 35706..36011 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag C2U49_RS31445 Protein ID WP_001568025.1
Coordinates 35486..35704 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U49_RS31415 31389..32666 - 1278 WP_103214963.1 HlyD family secretion protein -
C2U49_RS31420 33328..34206 + 879 WP_032427228.1 restriction endonuclease -
C2U49_RS31425 34247..34663 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
C2U49_RS31430 34660..34890 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C2U49_RS31445 35486..35704 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C2U49_RS31450 35706..36011 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
C2U49_RS31455 36180..36575 + 396 WP_017899885.1 hypothetical protein -
C2U49_RS31460 36602..36916 + 315 WP_053389906.1 hypothetical protein -
C2U49_RS31465 36927..37943 + 1017 WP_017899884.1 hypothetical protein -
C2U49_RS31470 38141..38935 + 795 WP_004197635.1 site-specific integrase -
C2U49_RS34520 39367..39669 - 303 WP_071571079.1 hypothetical protein -
C2U49_RS31480 39666..40292 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40784 40784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T93679 WP_013023785.1 NZ_CP026185:35706-36011 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T93679 NZ_CP026185:35706-36011 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT93679 WP_001568025.1 NZ_CP026185:35486-35704 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT93679 NZ_CP026185:35486-35704 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References