Detailed information of TA system TA093676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4925..5450
• Replicon: plasmid pKPN-0c4e
• Accession: NZ_CP026182
• Organism: Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: C2U49_RS30485
• Protein ID: WP_016946795.1
• Coordinates: 5145..5450 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: C2U49_RS30480
• Protein ID: WP_004197642.1
• Coordinates: 4925..5143 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U49_RS30430	301..504	-	204	WP_004197809.1	hypothetical protein	-
C2U49_RS30435	518..721	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
C2U49_RS30440	755..1123	-	369	WP_004197807.1	hypothetical protein	-
C2U49_RS30445	1167..1661	-	495	WP_020802777.1	hypothetical protein	-
C2U49_RS30450	1692..2267	-	576	WP_020317495.1	hypothetical protein	-
C2U49_RS30455	2255..2524	-	270	WP_020804424.1	hypothetical protein	-
C2U49_RS30460	2961..3650	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
C2U49_RS30465	3682..4371	-	690	WP_004193995.1	hypothetical protein	-
C2U49_RS30480	4925..5143	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C2U49_RS30485	5145..5450	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
C2U49_RS30490	5608..6291	+	684	WP_023280892.1	hypothetical protein	-
C2U49_RS30495	6294..7070	+	777	WP_049245386.1	site-specific integrase	-
C2U49_RS30500	7128..7385	-	258	WP_000764642.1	hypothetical protein	-
C2U49_RS34500	7514..7618	-	105	WP_032409716.1	hypothetical protein	-
C2U49_RS30505	8153..9019	+	867	WP_004118283.1	replication initiation protein	-
C2U49_RS30510	9376..9645	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..61178	61178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T93676 WP_016946795.1 NZ_CP026182:5145-5450 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T93676 NZ_CP026182:5145-5450 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT93676 WP_004197642.1 NZ_CP026182:4925-5143 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT93676 NZ_CP026182:4925-5143 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA