Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 204801..205444 Replicon plasmid pKPC-224e
Accession NZ_CP026179
Organism Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2U49_RS28955 Protein ID WP_001044770.1
Coordinates 205028..205444 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2U49_RS28950 Protein ID WP_001261282.1
Coordinates 204801..205031 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U49_RS28920 200923..201558 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
C2U49_RS28925 201666..202007 - 342 WP_071844672.1 hypothetical protein -
C2U49_RS28930 202330..203266 + 937 Protein_222 CAAX protease -
C2U49_RS28935 203457..203897 - 441 WP_047057603.1 hypothetical protein -
C2U49_RS28940 203916..204509 - 594 WP_009309909.1 hypothetical protein -
C2U49_RS34480 204608..204844 - 237 Protein_225 hypothetical protein -
C2U49_RS28950 204801..205031 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2U49_RS28955 205028..205444 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2U49_RS28960 205518..207077 + 1560 WP_103215780.1 AAA family ATPase -
C2U49_RS28965 207119..208087 - 969 WP_072269234.1 IS5 family transposase -
C2U49_RS28970 208131..209153 + 1023 WP_000361404.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaKPC-2 / qnrB19 / blaSHV-12 - 1..237571 237571
- flank IS/Tn - - 207119..208087 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93675 WP_001044770.1 NZ_CP026179:205028-205444 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93675 NZ_CP026179:205028-205444 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93675 WP_001261282.1 NZ_CP026179:204801-205031 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93675 NZ_CP026179:204801-205031 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References