Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 109244..109980 Replicon plasmid pKPC-224e
Accession NZ_CP026179
Organism Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag C2U49_RS28310 Protein ID WP_003026803.1
Coordinates 109244..109726 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag C2U49_RS28315 Protein ID WP_003026799.1
Coordinates 109714..109980 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U49_RS28295 105377..106009 + 633 WP_001567369.1 hypothetical protein -
C2U49_RS28300 106038..107441 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
C2U49_RS28305 107553..109085 + 1533 WP_009310015.1 IS3-like element ISKpn38 family transposase -
C2U49_RS28310 109244..109726 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
C2U49_RS28315 109714..109980 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
C2U49_RS28325 110425..110655 - 231 WP_004206660.1 hypothetical protein -
C2U49_RS28330 110669..110872 - 204 WP_004206661.1 hemolysin expression modulator Hha -
C2U49_RS28335 110933..111424 - 492 WP_004206662.1 hypothetical protein -
C2U49_RS28340 111558..112769 - 1212 WP_032415728.1 hypothetical protein -
C2U49_RS28345 112766..113014 - 249 WP_004193994.1 hypothetical protein -
C2U49_RS28350 113372..113722 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
C2U49_RS28355 113774..114136 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaKPC-2 / qnrB19 / blaSHV-12 - 1..237571 237571


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T93672 WP_003026803.1 NZ_CP026179:c109726-109244 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T93672 NZ_CP026179:c109726-109244 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT93672 WP_003026799.1 NZ_CP026179:c109980-109714 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT93672 NZ_CP026179:c109980-109714 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References