Detailed information of TA system TA093654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1000432..1002020
• Replicon: chromosome
• Accession: NZ_CP026178
• Organism: Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: C2U49_RS04990
• Protein ID: WP_002904792.1
• Coordinates: 1000698..1002020 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: C2U49_RS04985
• Protein ID: WP_004176198.1
• Coordinates: 1000432..1000698 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U49_RS04960	995461..996384	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
C2U49_RS04965	996365..997519	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
C2U49_RS04970	997865..999244	+	1380	WP_004176204.1	purine permease	-
C2U49_RS04975	999222..999722	+	501	WP_048981964.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
C2U49_RS04980	999719..1000045	+	327	WP_103215400.1	hydroxyisourate hydrolase	-
C2U49_RS04985	1000432..1000698	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
C2U49_RS04990	1000698..1002020	+	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
C2U49_RS04995	1002037..1003329	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
C2U49_RS05005	1003471..1004475	-	1005	WP_004184029.1	type 1 fimbrial protein	-
C2U49_RS05010	1004463..1006964	-	2502	WP_048981966.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaSHV-187	iroE / mgtC	743894..1144214	400320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T93654 WP_002904792.1 NZ_CP026178:1000698-1002020 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T93654 NZ_CP026178:1000698-1002020 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACACTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT93654 WP_004176198.1 NZ_CP026178:1000432-1000698 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT93654 NZ_CP026178:1000432-1000698 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8