Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3654700..3655319 Replicon chromosome
Accession NZ_CP026177
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C2U50_RS22590 Protein ID WP_002892050.1
Coordinates 3654700..3654918 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C2U50_RS22595 Protein ID WP_002892066.1
Coordinates 3654945..3655319 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS22560 3650747..3651010 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
C2U50_RS22565 3651010..3651150 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C2U50_RS22570 3651147..3651845 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
C2U50_RS22575 3651946..3653397 + 1452 WP_040236504.1 PLP-dependent aminotransferase family protein -
C2U50_RS22580 3653372..3653842 - 471 WP_002892026.1 YlaC family protein -
C2U50_RS33440 3653863..3654003 + 141 WP_004147370.1 hypothetical protein -
C2U50_RS22585 3653975..3654541 - 567 WP_002892030.1 maltose O-acetyltransferase -
C2U50_RS22590 3654700..3654918 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C2U50_RS22595 3654945..3655319 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C2U50_RS22600 3655805..3658951 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C2U50_RS22605 3658974..3660167 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T93644 WP_002892050.1 NZ_CP026177:c3654918-3654700 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T93644 NZ_CP026177:c3654918-3654700 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT93644 WP_002892066.1 NZ_CP026177:c3655319-3654945 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT93644 NZ_CP026177:c3655319-3654945 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References