Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 162948..163473 Replicon plasmid pKPC-0cc9
Accession NZ_CP026175
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag C2U50_RS03555 Protein ID WP_004197633.1
Coordinates 163168..163473 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag C2U50_RS03550 Protein ID WP_006788213.1
Coordinates 162948..163166 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS03520 158850..160127 - 1278 WP_103214963.1 HlyD family secretion protein -
C2U50_RS03525 160789..161667 + 879 WP_032427228.1 restriction endonuclease -
C2U50_RS03530 161708..162124 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
C2U50_RS03535 162121..162351 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C2U50_RS03550 162948..163166 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C2U50_RS03555 163168..163473 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
C2U50_RS03560 163531..163842 + 312 WP_009309919.1 hypothetical protein -
C2U50_RS03565 163892..164227 + 336 WP_009309920.1 hypothetical protein -
C2U50_RS03570 164261..165277 + 1017 WP_009309921.1 hypothetical protein -
C2U50_RS03575 165475..166254 + 780 WP_023287113.1 site-specific integrase -
C2U50_RS03580 166312..166569 - 258 WP_009310077.1 hypothetical protein -
C2U50_RS33095 166698..166811 - 114 WP_014343462.1 hypothetical protein -
C2U50_RS03585 166914..167894 + 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..172259 172259


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T93635 WP_004197633.1 NZ_CP026175:163168-163473 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T93635 NZ_CP026175:163168-163473 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT93635 WP_006788213.1 NZ_CP026175:162948-163166 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT93635 NZ_CP026175:162948-163166 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References