Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 152231..152874 Replicon plasmid pKPC-0cc9
Accession NZ_CP026175
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2U50_RS03475 Protein ID WP_001044770.1
Coordinates 152231..152647 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2U50_RS03480 Protein ID WP_001261282.1
Coordinates 152644..152874 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS03455 147599..147949 + 351 WP_004187110.1 DUF305 domain-containing protein -
C2U50_RS03460 148135..149043 - 909 WP_032451458.1 HNH endonuclease -
C2U50_RS03465 149588..150610 - 1023 WP_000361404.1 helicase UvrD -
C2U50_RS03470 150595..152157 - 1563 WP_009309907.1 AAA family ATPase -
C2U50_RS03475 152231..152647 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2U50_RS03480 152644..152874 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2U50_RS33090 152831..153067 + 237 Protein_181 hypothetical protein -
C2U50_RS03490 153166..153759 + 594 WP_009309909.1 hypothetical protein -
C2U50_RS03495 153778..154218 + 441 WP_047057603.1 hypothetical protein -
C2U50_RS03500 154409..155345 - 937 Protein_184 CAAX protease -
C2U50_RS03505 155668..156009 + 342 WP_071844672.1 hypothetical protein -
C2U50_RS03510 156117..156752 - 636 WP_009309912.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..172259 172259
- flank IS/Tn - - 146550..147518 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93633 WP_001044770.1 NZ_CP026175:c152647-152231 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93633 NZ_CP026175:c152647-152231 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93633 WP_001261282.1 NZ_CP026175:c152874-152644 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93633 NZ_CP026175:c152874-152644 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References