Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 118194..118930 Replicon plasmid pKPC-0cc9
Accession NZ_CP026175
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag C2U50_RS03285 Protein ID WP_003026803.1
Coordinates 118194..118676 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag C2U50_RS03290 Protein ID WP_003026799.1
Coordinates 118664..118930 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS03260 113400..113771 + 372 WP_003100853.1 hypothetical protein -
C2U50_RS03265 113765..114322 + 558 WP_003100847.1 recombinase family protein -
C2U50_RS03270 114401..115405 + 1005 WP_049247875.1 IS110-like element IS4321 family transposase -
C2U50_RS03275 115929..116561 + 633 WP_001567369.1 hypothetical protein -
C2U50_RS03280 116590..117993 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
C2U50_RS03285 118194..118676 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
C2U50_RS03290 118664..118930 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
C2U50_RS03295 119106..119360 - 255 WP_004118702.1 hypothetical protein -
C2U50_RS03300 119436..119693 - 258 WP_004098928.1 hypothetical protein -
C2U50_RS03305 119742..119945 - 204 WP_004098931.1 hemolysin expression modulator Hha -
C2U50_RS03310 119976..120344 - 369 WP_103214962.1 hypothetical protein -
C2U50_RS03315 120388..120882 - 495 WP_009310053.1 hypothetical protein -
C2U50_RS03320 120913..121479 - 567 WP_009310052.1 hypothetical protein -
C2U50_RS03325 121476..121739 - 264 WP_009310051.1 hypothetical protein -
C2U50_RS03330 122029..123567 - 1539 WP_004196907.1 IS66-like element ISEc22 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..172259 172259
- inside IScluster/Tn blaKPC-2 - 100832..124364 23532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T93632 WP_003026803.1 NZ_CP026175:c118676-118194 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T93632 NZ_CP026175:c118676-118194 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT93632 WP_003026799.1 NZ_CP026175:c118930-118664 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT93632 NZ_CP026175:c118930-118664 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References