Detailed information of TA system TA093627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 150102..150772
• Replicon: plasmid pF81_2
• Accession: NZ_CP026166
• Organism: Klebsiella pneumoniae strain F81

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C2M15_RS27025
• Protein ID: WP_004213072.1
• Coordinates: 150102..150545 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C2M15_RS27030
• Protein ID: WP_004213073.1
• Coordinates: 150542..150772 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M15_RS26975	145512..145787	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
C2M15_RS26980	145850..146341	+	492	WP_011251327.1	DM13 domain-containing protein	-
C2M15_RS26985	146390..147310	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
C2M15_RS26995	147401..147807	+	407	Protein_154	GAF domain-containing protein	-
C2M15_RS28385	148322..148958	-	637	Protein_155	mucoid phenotype A regulator RmpA	-
C2M15_RS27015	149375..149668	+	294	Protein_156	transposase	-
C2M15_RS27020	149672..150088	+	417	Protein_157	restriction endonuclease	-
C2M15_RS27025	150102..150545	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2M15_RS27030	150542..150772	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2M15_RS27035	151380..152513	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C2M15_RS27040	152529..152822	+	294	WP_004213076.1	hypothetical protein	-
C2M15_RS27045	152812..153018	-	207	WP_004213077.1	hypothetical protein	-
C2M15_RS27050	153370..153660	+	291	WP_004213078.1	hypothetical protein	-
C2M15_RS27055	153650..154549	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..211437	211437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T93627 WP_004213072.1 NZ_CP026166:c150545-150102 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T93627 NZ_CP026166:c150545-150102 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT93627 WP_004213073.1 NZ_CP026166:c150772-150542 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT93627 NZ_CP026166:c150772-150542 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620