Detailed information of TA system TA093625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 57458..58194
• Replicon: plasmid pF81_2
• Accession: NZ_CP026166
• Organism: Klebsiella pneumoniae strain F81

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C2M15_RS26490
• Protein ID: WP_117985291.1
• Coordinates: 57712..58194 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: C2M15_RS26485
• Protein ID: WP_004213599.1
• Coordinates: 57458..57724 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M15_RS26460	52690..53247	+	558	WP_129073998.1	recombinase family protein	-
C2M15_RS26465	53250..56219	+	2970	WP_004213592.1	Tn3 family transposase	-
C2M15_RS26470	56261..56755	+	495	WP_004213594.1	hypothetical protein	-
C2M15_RS26475	56816..57019	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
C2M15_RS26480	57033..57263	+	231	WP_004213598.1	hypothetical protein	-
C2M15_RS26485	57458..57724	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
C2M15_RS26490	57712..58194	+	483	WP_117985291.1	GNAT family N-acetyltransferase	Toxin
C2M15_RS26495	58615..59842	+	1228	Protein_58	IS3 family transposase	-
C2M15_RS26500	59838..60233	-	396	Protein_59	DDE-type integrase/transposase/recombinase	-
C2M15_RS26505	60408..61430	-	1023	WP_004214536.1	porphobilinogen synthase	-
C2M15_RS26510	61494..62840	-	1347	WP_048333011.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..211437	211437
	-	inside	IScluster/Tn	-	-	52690..59842	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T93625 WP_117985291.1 NZ_CP026166:57712-58194 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENLGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T93625 NZ_CP026166:57712-58194 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACCTTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT93625 WP_004213599.1 NZ_CP026166:57458-57724 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT93625 NZ_CP026166:57458-57724 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0