Detailed information of TA system TA093622

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4534694..4535385
• Replicon: chromosome
• Accession: NZ_CP026164
• Organism: Klebsiella pneumoniae strain F81

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: C2M15_RS22450
• Protein ID: WP_129073948.1
• Coordinates: 4534694..4535035 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2A5MHA6
• Locus tag: C2M15_RS22455
• Protein ID: WP_019725272.1
• Coordinates: 4535059..4535385 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M15_RS22415	4529917..4530147	-	231	WP_077256410.1	hypothetical protein	-
C2M15_RS28465	4530216..4530404	-	189	WP_077256411.1	hypothetical protein	-
C2M15_RS22425	4530857..4531549	-	693	WP_040173594.1	HNH endonuclease	-
C2M15_RS22430	4531746..4532328	+	583	Protein_4322	tyrosine-type recombinase/integrase	-
C2M15_RS22435	4532469..4533377	+	909	WP_077256408.1	hypothetical protein	-
C2M15_RS22440	4533456..4533740	+	285	Protein_4324	helix-turn-helix domain-containing protein	-
C2M15_RS22445	4533746..4534579	-	834	WP_040173641.1	DUF4942 domain-containing protein	-
C2M15_RS22450	4534694..4535035	-	342	WP_129073948.1	TA system toxin CbtA family protein	Toxin
C2M15_RS22455	4535059..4535385	-	327	WP_019725272.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C2M15_RS22460	4535399..4535875	-	477	WP_019725273.1	DNA repair protein RadC	-
C2M15_RS22465	4535885..4536331	-	447	WP_040173599.1	antirestriction protein	-
C2M15_RS22470	4536542..4537231	-	690	WP_009309812.1	hypothetical protein	-
C2M15_RS22480	4537796..4538686	-	891	WP_085163516.1	50S ribosome-binding GTPase	-
C2M15_RS22485	4538769..4539857	-	1089	WP_085163515.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4517154..4546145	28991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12885.93 Da
• Isoelectric Point: 8.5195

>T93622 WP_129073948.1 NZ_CP026164:c4535035-4534694 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTMNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T93622 NZ_CP026164:c4535035-4534694 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCATGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12276.01 Da
• Isoelectric Point: 6.9560

>AT93622 WP_019725272.1 NZ_CP026164:c4535385-4535059 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT93622 NZ_CP026164:c4535385-4535059 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGGGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MHA6