Detailed information of TA system TA093605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 19570..20330
• Replicon: plasmid pF13_1
• Accession: NZ_CP026163
• Organism: Klebsiella pneumoniae strain F13

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C2M13_RS26830
• Protein ID: WP_064402685.1
• Coordinates: 19570..20055 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C2M13_RS26835
• Protein ID: WP_086531840.1
• Coordinates: 20043..20330 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M13_RS26780	15171..15584	-	414	WP_004182074.1	helix-turn-helix domain-containing protein	-
C2M13_RS26785	15585..15863	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
C2M13_RS26790	15853..16173	-	321	WP_004152720.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2M13_RS26795	16254..16478	-	225	WP_004152719.1	hypothetical protein	-
C2M13_RS26800	16489..16701	-	213	WP_004152718.1	hypothetical protein	-
C2M13_RS26805	16762..17118	-	357	WP_004152717.1	hypothetical protein	-
C2M13_RS26815	17754..18104	-	351	WP_023320095.1	hypothetical protein	-
C2M13_RS26820	18101..18373	-	273	WP_004182067.1	hypothetical protein	-
C2M13_RS26825	19069..19230	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
C2M13_RS26830	19570..20055	-	486	WP_064402685.1	GNAT family N-acetyltransferase	Toxin
C2M13_RS26835	20043..20330	-	288	WP_086531840.1	DUF1778 domain-containing protein	Antitoxin
C2M13_RS26840	20556..21707	-	1152	WP_063434792.1	MFS transporter	-
C2M13_RS26845	22031..22777	+	747	WP_074165104.1	IclR family transcriptional regulator	-
C2M13_RS26850	22792..23010	-	219	Protein_36	IS1 family transposase	-
C2M13_RS26860	24709..25083	-	375	WP_023320094.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120075	120075
	-	flank	IS/Tn	-	-	23141..23944	803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17791.51 Da
• Isoelectric Point: 9.4841

>T93605 WP_064402685.1 NZ_CP026163:c20055-19570 [Klebsiella pneumoniae]
VGRVTAPEPLSPYHQVAEFVSGETVLDDWLKQKGLKNQALGAARTFVVCKKDTQQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T93605 NZ_CP026163:c20055-19570 [Klebsiella pneumoniae]
GTGGGACGTGTAACAGCACCAGAACCTCTTTCCCCTTATCATCAGGTGGCTGAGTTCGTCAGTGGTGAGACTGTGCTCGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGAGCGGCCCGAACGTTTGTGGTGTGTAAGAAGGACACTC
AGCAAATTGCTGGTTTTTACTCACTGGCAACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGCCGTAACATG
CCAGATCCCATTCCTGTCATTATACTAGCCCGTCTTGCCGTCGATCTCTCATTCCGCGGGAAAGGGCTTGGCGCTGATTT
ACTTCATGACGCAGTGCTTCGTTGCTATCGTGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCGAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGGAACGGACATTATTCCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 4.6696

>AT93605 WP_086531840.1 NZ_CP026163:c20330-20043 [Klebsiella pneumoniae]
MLYKRCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDD
PVIEKLLVRKPQWDV

Nucleotide

• Length: 288 bp

>AT93605 NZ_CP026163:c20330-20043 [Klebsiella pneumoniae]
ATGCTATACAAGAGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGATATGCTTGATGCACCCGTTACAGATGAT
CCCGTTATCGAAAAACTGCTGGTAAGGAAACCTCAGTGGGACGTGTAA