Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4072306..4072925 Replicon chromosome
Accession NZ_CP026162
Organism Klebsiella pneumoniae strain F13

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C2M13_RS20285 Protein ID WP_002892050.1
Coordinates 4072707..4072925 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C2M13_RS20280 Protein ID WP_002892066.1
Coordinates 4072306..4072680 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M13_RS20270 4067458..4068651 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C2M13_RS20275 4068674..4071820 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C2M13_RS20280 4072306..4072680 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C2M13_RS20285 4072707..4072925 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C2M13_RS20290 4073084..4073650 + 567 WP_002892030.1 maltose O-acetyltransferase -
C2M13_RS28165 4073622..4073762 - 141 WP_004147370.1 hypothetical protein -
C2M13_RS20295 4073783..4074253 + 471 WP_002892026.1 YlaC family protein -
C2M13_RS20300 4074228..4075679 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
C2M13_RS20305 4075780..4076478 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
C2M13_RS20310 4076475..4076615 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C2M13_RS20315 4076615..4076878 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T93600 WP_002892050.1 NZ_CP026162:4072707-4072925 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T93600 NZ_CP026162:4072707-4072925 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT93600 WP_002892066.1 NZ_CP026162:4072306-4072680 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT93600 NZ_CP026162:4072306-4072680 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References