Detailed information of TA system TA093586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 22919..23658
• Replicon: plasmid pF93-1_1
• Accession: NZ_CP026161
• Organism: Klebsiella pneumoniae strain F93-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: C2M21_RS26025
• Protein ID: WP_021312536.1
• Coordinates: 22919..23404 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: C2M21_RS26030
• Protein ID: WP_003026799.1
• Coordinates: 23392..23658 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M21_RS25980	17965..18381	-	417	WP_074193590.1	relaxosome protein TraM	-
C2M21_RS25985	18772..19257	+	486	WP_001568108.1	transglycosylase SLT domain-containing protein	-
C2M21_RS25990	19290..19619	-	330	WP_074193592.1	hypothetical protein	-
C2M21_RS25995	19652..20473	-	822	WP_064081511.1	DUF945 domain-containing protein	-
C2M21_RS26005	21101..21451	-	351	WP_064081512.1	hypothetical protein	-
C2M21_RS26010	21448..21720	-	273	WP_032445785.1	hypothetical protein	-
C2M21_RS26020	22415..22576	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
C2M21_RS26025	22919..23404	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
C2M21_RS26030	23392..23658	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
C2M21_RS26035	23791..24219	-	429	WP_004901287.1	GFA family protein	-
C2M21_RS26040	24420..25788	-	1369	Protein_37	IS3 family transposase	-
C2M21_RS26050	25994..26233	-	240	WP_064081514.1	hypothetical protein	-
C2M21_RS26060	26457..27188	-	732	WP_064279160.1	plasmid SOS inhibition protein A	-
C2M21_RS26065	27185..27616	-	432	WP_004194235.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..165465	165465
	-	flank	IS/Tn	-	-	24420..25145	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T93586 WP_021312536.1 NZ_CP026161:c23404-22919 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T93586 NZ_CP026161:c23404-22919 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT93586 WP_003026799.1 NZ_CP026161:c23658-23392 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT93586 NZ_CP026161:c23658-23392 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66