Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5112924..5113549 Replicon chromosome
Accession NZ_CP026160
Organism Klebsiella pneumoniae strain F93-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag C2M21_RS25450 Protein ID WP_002882817.1
Coordinates 5112924..5113307 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C2M21_RS25455 Protein ID WP_004150355.1
Coordinates 5113307..5113549 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M21_RS25435 5110290..5111192 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C2M21_RS25440 5111189..5111824 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C2M21_RS25445 5111821..5112750 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C2M21_RS25450 5112924..5113307 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
C2M21_RS25455 5113307..5113549 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
C2M21_RS25460 5113754..5114671 + 918 WP_004181612.1 alpha/beta hydrolase -
C2M21_RS25465 5114685..5115626 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C2M21_RS25470 5115671..5116108 - 438 WP_101986966.1 D-tyrosyl-tRNA(Tyr) deacylase -
C2M21_RS25475 5116105..5116965 - 861 WP_004146232.1 virulence factor BrkB family protein -
C2M21_RS25480 5116959..5117558 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T93585 WP_002882817.1 NZ_CP026160:c5113307-5112924 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T93585 NZ_CP026160:c5113307-5112924 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT93585 WP_004150355.1 NZ_CP026160:c5113549-5113307 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT93585 NZ_CP026160:c5113549-5113307 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References