Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2836316..2837052 Replicon chromosome
Accession NZ_CP026155
Organism Klebsiella pneumoniae strain B12(AN)

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag C2M23_RS14250 Protein ID WP_003026803.1
Coordinates 2836316..2836798 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag C2M23_RS14255 Protein ID WP_003026799.1
Coordinates 2836786..2837052 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M23_RS14235 2831953..2832915 + 963 WP_004152113.1 M48 family metalloprotease -
C2M23_RS14240 2833003..2834257 + 1255 Protein_2740 IS3 family transposase -
C2M23_RS14245 2834758..2836104 - 1347 WP_020314316.1 ISNCY family transposase -
C2M23_RS14250 2836316..2836798 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
C2M23_RS14255 2836786..2837052 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
C2M23_RS14260 2837228..2837482 - 255 WP_004152108.1 hypothetical protein -
C2M23_RS14265 2837558..2837815 - 258 WP_004152107.1 hypothetical protein -
C2M23_RS14270 2837864..2838067 - 204 WP_004152106.1 hemolysin expression modulator Hha -
C2M23_RS14275 2838101..2838469 - 369 WP_004152105.1 hypothetical protein -
C2M23_RS14280 2838513..2839007 - 495 WP_004152104.1 DNA-binding protein -
C2M23_RS14285 2839038..2839613 - 576 WP_004152103.1 hypothetical protein -
C2M23_RS14290 2839601..2839870 - 270 WP_004152102.1 hypothetical protein -
C2M23_RS14295 2840228..2840578 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
C2M23_RS14300 2840628..2840990 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T93522 WP_003026803.1 NZ_CP026155:c2836798-2836316 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T93522 NZ_CP026155:c2836798-2836316 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT93522 WP_003026799.1 NZ_CP026155:c2837052-2836786 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT93522 NZ_CP026155:c2837052-2836786 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References