Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17561..18204 Replicon plasmid pF138_3
Accession NZ_CP026152
Organism Klebsiella pneumoniae strain F138

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2M09_RS29545 Protein ID WP_001044770.1
Coordinates 17561..17977 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2M09_RS29550 Protein ID WP_001261282.1
Coordinates 17974..18204 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M09_RS29525 12759..13640 - 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
C2M09_RS29530 13966..14663 - 698 Protein_20 IS1 family transposase -
C2M09_RS29535 14918..15940 - 1023 WP_000361404.1 helicase UvrD -
C2M09_RS29540 15925..17487 - 1563 WP_004206609.1 AAA family ATPase -
C2M09_RS29545 17561..17977 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2M09_RS29550 17974..18204 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2M09_RS29555 18161..18622 + 462 WP_014343465.1 hypothetical protein -
C2M09_RS29560 18783..19726 + 944 Protein_26 hypothetical protein -
C2M09_RS29565 19763..20155 + 393 WP_011977811.1 hypothetical protein -
C2M09_RS29570 20213..20734 + 522 WP_013214008.1 hypothetical protein -
C2M09_RS29575 20780..20983 + 204 WP_011977813.1 hypothetical protein -
C2M09_RS29580 21013..22017 + 1005 WP_011977814.1 hypothetical protein -
C2M09_RS29585 22201..22980 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / catA2 - 1..83260 83260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93503 WP_001044770.1 NZ_CP026152:c17977-17561 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93503 NZ_CP026152:c17977-17561 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93503 WP_001261282.1 NZ_CP026152:c18204-17974 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93503 NZ_CP026152:c18204-17974 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References