Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68408..69051 Replicon plasmid pF132_2
Accession NZ_CP026147
Organism Klebsiella pneumoniae strain F132

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2M08_RS28885 Protein ID WP_001044770.1
Coordinates 68408..68824 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2M08_RS28890 Protein ID WP_001261282.1
Coordinates 68821..69051 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2M08_RS28850 63789..64550 - 762 WP_002210513.1 DeoR/GlpR family DNA-binding transcription regulator -
C2M08_RS28860 64812..65713 + 902 Protein_83 NAD(P)-dependent oxidoreductase -
C2M08_RS28865 65725..66977 + 1253 Protein_84 four-carbon acid sugar kinase family protein -
C2M08_RS28870 66970..67293 + 324 Protein_85 class II aldolase/adducin family protein -
C2M08_RS28875 67347..68051 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
C2M08_RS28880 68098..68334 - 237 WP_001348075.1 AAA family ATPase -
C2M08_RS28885 68408..68824 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2M08_RS28890 68821..69051 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2M08_RS28895 69008..69469 + 462 WP_014343465.1 hypothetical protein -
C2M08_RS28900 69630..70574 + 945 WP_011977810.1 hypothetical protein -
C2M08_RS28905 70611..71003 + 393 WP_011977811.1 hypothetical protein -
C2M08_RS28910 71061..71582 + 522 WP_013214008.1 hypothetical protein -
C2M08_RS28915 71628..71831 + 204 WP_011977813.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / catA2 / blaKPC-2 / blaSHV-12 - 1..71854 71854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93483 WP_001044770.1 NZ_CP026147:c68824-68408 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93483 NZ_CP026147:c68824-68408 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93483 WP_001261282.1 NZ_CP026147:c69051-68821 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93483 NZ_CP026147:c69051-68821 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References