Detailed information of TA system TA093461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 249485..250121
• Replicon: chromosome
• Accession: NZ_CP026143
• Organism: Bacillus methanolicus strain DFS2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: I3E3S0
• Locus tag: C0971_RS01370
• Protein ID: WP_003348433.1
• Coordinates: 249771..250121 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: C0971_RS01365
• Protein ID: WP_248892689.1
• Coordinates: 249485..249766 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0971_RS01345 (C0971_01350)	245312..245911	-	600	WP_003348445.1	rhomboid family intramembrane serine protease	-
C0971_RS01350 (C0971_01355)	246030..246386	+	357	WP_003348443.1	holo-ACP synthase	-
C0971_RS01355 (C0971_01360)	246588..247601	+	1014	WP_034669513.1	outer membrane lipoprotein carrier protein LolA	-
C0971_RS01360 (C0971_01365)	247884..249047	+	1164	WP_003348440.1	alanine racemase	-
C0971_RS01365 (C0971_01375)	249485..249766	+	282	WP_248892689.1	CopG family ribbon-helix-helix protein	Antitoxin
C0971_RS01370 (C0971_01380)	249771..250121	+	351	WP_003348433.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C0971_RS01375 (C0971_01385)	250457..251278	+	822	WP_248892690.1	RsbT co-antagonist protein RsbRA	-
C0971_RS01380 (C0971_01390)	251275..251631	+	357	WP_003348429.1	STAS domain-containing protein	-
C0971_RS01385 (C0971_01395)	251636..252037	+	402	WP_003348427.1	anti-sigma regulatory factor	-
C0971_RS01390 (C0971_01400)	252048..253058	+	1011	WP_003348425.1	PP2C family protein-serine/threonine phosphatase	-
C0971_RS01395 (C0971_01405)	253115..253444	+	330	WP_003348423.1	anti-sigma factor antagonist	-
C0971_RS01400 (C0971_01410)	253444..253917	+	474	WP_003348421.1	anti-sigma B factor RsbW	-
C0971_RS01405 (C0971_01415)	253895..254689	+	795	WP_003348419.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13022.03 Da
• Isoelectric Point: 4.8998

>T93461 WP_003348433.1 NZ_CP026143:249771-250121 [Bacillus methanolicus]
MIVKRGDVYFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T93461 NZ_CP026143:249771-250121 [Bacillus methanolicus]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCGGACCTATCCCCAGTTGTTGGGTCAGAACAAGGCGGAACCCGTCCTGT
ACTAGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTTATCGTAGCAGCGATCACTGCCCAAATCCAAAAAG
CAAAGCTGCCCACACACGTTGAAATTGATGCGAAACGTTACGGTTTTGAACGAGACTCTGTCATCCTATTGGAACAAATT
CGTACAATTGATAAACAGCGATTAACCGATAAGATTACTCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCCT
TCAAATCAGTTTAGGTCTCATAGAGTTCTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10730.19 Da
• Isoelectric Point: 7.2028

>AT93461 WP_248892689.1 NZ_CP026143:249485-249766 [Bacillus methanolicus]
VSESSATTEILVKLPQHLLNELDGFVKQENVNRSELIYQATKAYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT93461 NZ_CP026143:249485-249766 [Bacillus methanolicus]
GTGTCTGAGTCCAGTGCAACAACGGAGATCTTGGTAAAATTACCGCAACATCTTTTAAACGAATTAGATGGTTTTGTAAA
ACAAGAAAATGTAAATCGCAGCGAACTTATTTATCAAGCAACAAAAGCGTATTTGCGTGAACGTAAAAAGAGACAAATTC
GTGAATCAATGAGGCGCGGCTATATGGAAATGGCCAAAATTAATCTGACGATTGCATCAGAAGCATTCCAAGCAGAATAT
GAGGCAGAACACACAGTTGAACGTCTAGTTAGCGGGGGATAA

Structures

Toxin

• AlphaFold DB: I3E3S0