Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 131336..131979 Replicon plasmid pF127_1
Accession NZ_CP026141
Organism Klebsiella pneumoniae strain F127

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2L73_RS28860 Protein ID WP_001044770.1
Coordinates 131563..131979 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2L73_RS28855 Protein ID WP_001261282.1
Coordinates 131336..131566 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2L73_RS28820 126559..127338 - 780 WP_013214009.1 site-specific integrase -
C2L73_RS28825 127522..128526 - 1005 WP_011977814.1 hypothetical protein -
C2L73_RS28830 128556..128759 - 204 WP_011977813.1 hypothetical protein -
C2L73_RS28835 128805..129326 - 522 WP_013214008.1 hypothetical protein -
C2L73_RS28840 129384..129776 - 393 WP_011977811.1 hypothetical protein -
C2L73_RS28845 129813..130757 - 945 WP_011977810.1 hypothetical protein -
C2L73_RS28850 130918..131379 - 462 WP_014343465.1 hypothetical protein -
C2L73_RS28855 131336..131566 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2L73_RS28860 131563..131979 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2L73_RS28865 132053..133615 + 1563 WP_004206609.1 AAA family ATPase -
C2L73_RS28870 133600..134622 + 1023 WP_000361404.1 helicase UvrD -
C2L73_RS28875 134878..135575 + 698 Protein_183 IS1 family transposase -
C2L73_RS28880 135901..136782 + 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-65 / catA2 / blaKPC-2 / blaSHV-12 - 1..164501 164501


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93460 WP_001044770.1 NZ_CP026141:131563-131979 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93460 NZ_CP026141:131563-131979 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93460 WP_001261282.1 NZ_CP026141:131336-131566 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93460 NZ_CP026141:131336-131566 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References