Detailed information of TA system TA093433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 87557..88200
• Replicon: plasmid pF77_1
• Accession: NZ_CP026137
• Organism: Klebsiella pneumoniae strain F77

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: C2L62_RS28560
• Protein ID: WP_001044770.1
• Coordinates: 87784..88200 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: C2L62_RS28555
• Protein ID: WP_001261282.1
• Coordinates: 87557..87787 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2L62_RS28520	82780..83559	-	780	WP_013214009.1	site-specific integrase	-
C2L62_RS28525	83743..84747	-	1005	WP_011977814.1	hypothetical protein	-
C2L62_RS28530	84777..84980	-	204	WP_011977813.1	hypothetical protein	-
C2L62_RS28535	85026..85547	-	522	WP_013214008.1	hypothetical protein	-
C2L62_RS28540	85605..85997	-	393	WP_011977811.1	hypothetical protein	-
C2L62_RS28545	86034..86978	-	945	WP_011977810.1	hypothetical protein	-
C2L62_RS28550	87139..87600	-	462	WP_014343465.1	hypothetical protein	-
C2L62_RS28555	87557..87787	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2L62_RS28560	87784..88200	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2L62_RS28565	88274..88510	+	237	WP_001348075.1	AAA family ATPase	-
C2L62_RS28570	88557..89261	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C2L62_RS28575	89315..89638	-	324	Protein_119	class II aldolase/adducin family protein	-
C2L62_RS28580	89631..90883	-	1253	Protein_120	four-carbon acid sugar kinase family protein	-
C2L62_RS28585	90895..91797	-	903	WP_002903955.1	NAD(P)-dependent oxidoreductase	-
C2L62_RS28595	92059..92820	+	762	WP_002210513.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA2 / blaKPC-2 / blaSHV-12 / blaCTX-M-65 / fosA3	-	1..104887	104887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T93433 WP_001044770.1 NZ_CP026137:87784-88200 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T93433 NZ_CP026137:87784-88200 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT93433 WP_001261282.1 NZ_CP026137:87557-87787 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT93433 NZ_CP026137:87557-87787 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3