Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 9574..10310 Replicon plasmid pF5_3
Accession NZ_CP026135
Organism Klebsiella pneumoniae strain F5

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag C2L61_RS29395 Protein ID WP_003026803.1
Coordinates 9574..10056 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag C2L61_RS29400 Protein ID WP_003026799.1
Coordinates 10044..10310 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2L61_RS29380 5211..6173 + 963 WP_004152113.1 M48 family metalloprotease -
C2L61_RS29385 6261..7515 + 1255 Protein_7 IS3 family transposase -
C2L61_RS29390 8016..9362 - 1347 WP_020314316.1 ISNCY family transposase -
C2L61_RS29395 9574..10056 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
C2L61_RS29400 10044..10310 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
C2L61_RS29405 10486..10740 - 255 WP_004152108.1 hypothetical protein -
C2L61_RS29410 10816..11073 - 258 WP_004152107.1 hypothetical protein -
C2L61_RS29415 11122..11325 - 204 WP_004152106.1 hemolysin expression modulator Hha -
C2L61_RS29420 11359..11727 - 369 WP_004152105.1 hypothetical protein -
C2L61_RS29425 11771..12265 - 495 WP_004152104.1 DNA-binding protein -
C2L61_RS29430 12296..12871 - 576 WP_004152103.1 hypothetical protein -
C2L61_RS29435 12859..13128 - 270 WP_004152102.1 hypothetical protein -
C2L61_RS29440 13486..13836 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
C2L61_RS29445 13886..14248 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / catA2 - 1..161567 161567


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T93410 WP_003026803.1 NZ_CP026135:c10056-9574 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T93410 NZ_CP026135:c10056-9574 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT93410 WP_003026799.1 NZ_CP026135:c10310-10044 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT93410 NZ_CP026135:c10310-10044 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References