Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49054..49697 Replicon plasmid pF5_1
Accession NZ_CP026133
Organism Klebsiella pneumoniae strain F5

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2L61_RS28360 Protein ID WP_001044770.1
Coordinates 49281..49697 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2L61_RS28355 Protein ID WP_001261282.1
Coordinates 49054..49284 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2L61_RS28320 44277..45056 - 780 WP_013214009.1 site-specific integrase -
C2L61_RS28325 45240..46244 - 1005 WP_011977814.1 hypothetical protein -
C2L61_RS28330 46274..46477 - 204 WP_011977813.1 hypothetical protein -
C2L61_RS28335 46523..47044 - 522 WP_013214008.1 hypothetical protein -
C2L61_RS28340 47102..47494 - 393 WP_011977811.1 hypothetical protein -
C2L61_RS28345 47531..48475 - 945 WP_011977810.1 hypothetical protein -
C2L61_RS28350 48636..49097 - 462 WP_014343465.1 hypothetical protein -
C2L61_RS28355 49054..49284 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2L61_RS28360 49281..49697 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2L61_RS28365 49771..51333 + 1563 WP_004206609.1 AAA family ATPase -
C2L61_RS28370 51318..52340 + 1023 WP_000361404.1 helicase UvrD -
C2L61_RS28375 52596..53293 + 698 Protein_72 IS1 family transposase -
C2L61_RS28380 53619..54500 + 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / fosA3 / blaKPC-2 / blaSHV-12 / catA2 - 1..105124 105124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93406 WP_001044770.1 NZ_CP026133:49281-49697 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93406 NZ_CP026133:49281-49697 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93406 WP_001261282.1 NZ_CP026133:49054-49284 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93406 NZ_CP026133:49054-49284 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References