Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 65787..66430 Replicon plasmid pF1_1
Accession NZ_CP026131
Organism Klebsiella pneumoniae strain F1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C2L60_RS28565 Protein ID WP_001044770.1
Coordinates 66014..66430 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C2L60_RS28560 Protein ID WP_001261282.1
Coordinates 65787..66017 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2L60_RS28525 61010..61789 - 780 WP_013214009.1 site-specific integrase -
C2L60_RS28530 61973..62977 - 1005 WP_011977814.1 hypothetical protein -
C2L60_RS28535 63007..63210 - 204 WP_011977813.1 hypothetical protein -
C2L60_RS28540 63256..63777 - 522 WP_013214008.1 hypothetical protein -
C2L60_RS28545 63835..64227 - 393 WP_011977811.1 hypothetical protein -
C2L60_RS28550 64264..65208 - 945 WP_011977810.1 hypothetical protein -
C2L60_RS28555 65369..65830 - 462 WP_014343465.1 hypothetical protein -
C2L60_RS28560 65787..66017 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C2L60_RS28565 66014..66430 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C2L60_RS28570 66504..68066 + 1563 WP_004206609.1 AAA family ATPase -
C2L60_RS28575 68051..69073 + 1023 WP_000361404.1 helicase UvrD -
C2L60_RS28580 69329..70026 + 698 Protein_92 IS1 family transposase -
C2L60_RS28585 70352..71233 + 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65 - 1..164510 164510


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T93379 WP_001044770.1 NZ_CP026131:66014-66430 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T93379 NZ_CP026131:66014-66430 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT93379 WP_001261282.1 NZ_CP026131:65787-66017 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT93379 NZ_CP026131:65787-66017 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References