Detailed information of TA system TA093345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2035066..2036108
• Replicon: chromosome
• Accession: NZ_CP026122
• Organism: Aeromonas sp. ASNIH5

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: C2U30_RS09320
• Protein ID: WP_003109777.1
• Coordinates: 2035066..2035641 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: C2U30_RS09325
• Protein ID: WP_003050245.1
• Coordinates: 2035638..2036108 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U30_RS09295	2031504..2032229	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
C2U30_RS09300	2032268..2033170	-	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
C2U30_RS09305	2033170..2033670	-	501	WP_003090159.1	hypothetical protein	-
C2U30_RS09310	2033667..2034137	-	471	WP_003105626.1	hypothetical protein	-
C2U30_RS09315	2034134..2035048	-	915	WP_003105629.1	AAA family ATPase	-
C2U30_RS09320	2035066..2035641	-	576	WP_003109777.1	PIN domain-containing protein	Toxin
C2U30_RS09325	2035638..2036108	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
C2U30_RS09330	2036312..2036695	+	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
C2U30_RS09335	2036692..2036925	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
C2U30_RS09340	2036942..2037301	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
C2U30_RS09345	2037313..2037711	+	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
C2U30_RS09350	2037708..2038400	+	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
C2U30_RS09355	2038397..2039308	+	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
C2U30_RS09360	2039298..2040716	+	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1984060..2098046	113986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T93345 WP_003109777.1 NZ_CP026122:c2035641-2035066 [Aeromonas sp. ASNIH5]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T93345 NZ_CP026122:c2035641-2035066 [Aeromonas sp. ASNIH5]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT93345 WP_003050245.1 NZ_CP026122:c2036108-2035638 [Aeromonas sp. ASNIH5]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT93345 NZ_CP026122:c2036108-2035638 [Aeromonas sp. ASNIH5]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68