Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 147277..148034 Replicon plasmid unnamed
Accession NZ_CP026099
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name tacT Uniprot ID A0A896Z9U7
Locus tag MC63_RS24515 Protein ID WP_000501968.1
Coordinates 147277..147762 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag MC63_RS24520 Protein ID WP_011114751.1
Coordinates 147750..148034 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS25085 143494..144105 + 612 WP_141087860.1 hypothetical protein -
MC63_RS24495 (MC63_025620) 144145..145354 - 1210 Protein_181 IS3 family transposase -
MC63_RS24510 (MC63_025630) 146823..147104 + 282 Protein_183 IS4/IS5 family transposase -
MC63_RS24515 (MC63_025635) 147277..147762 - 486 WP_000501968.1 GNAT family N-acetyltransferase Toxin
MC63_RS24520 (MC63_025640) 147750..148034 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
MC63_RS25410 148935..149099 + 165 WP_001346193.1 hypothetical protein -
MC63_RS24540 (MC63_025665) 149353..150954 - 1602 WP_047564038.1 IS66-like element ISSfl3 family transposase -
MC63_RS24545 (MC63_025670) 150974..151321 - 348 WP_000631711.1 IS66 family insertion sequence element accessory protein TnpB -
MC63_RS24550 (MC63_025675) 151318..151992 - 675 WP_001088287.1 IS66-like element accessory protein TnpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospI / ipaH9.8 / ospG / ospC2 / icsP/sopA / ospB / ospC3 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ospC1 / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG 1..229413 229413
- inside IScluster/Tn - ospE2 / ipaH2.5 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ospC1 / ospD3/senA / ospC3 107996..160075 52079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17726.56 Da        Isoelectric Point: 10.1083

>T93312 WP_000501968.1 NZ_CP026099:c147762-147277 [Shigella flexneri]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T93312 NZ_CP026099:c147762-147277 [Shigella flexneri]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTATCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT93312 WP_011114751.1 NZ_CP026099:c148034-147750 [Shigella flexneri]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT93312 NZ_CP026099:c148034-147750 [Shigella flexneri]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896Z9U7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References