Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3129818..3130613 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name yeeV Uniprot ID Q93EZ7
Locus tag MC63_RS16150 Protein ID WP_000854917.1
Coordinates 3129818..3130192 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D2ACA1
Locus tag MC63_RS16155 Protein ID WP_001280958.1
Coordinates 3130239..3130613 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS16115 (3125428) 3125428..3126366 - 939 Protein_3087 glyoxylate/hydroxypyruvate reductase GhrA -
MC63_RS16125 (3126837) 3126837..3127118 - 282 Protein_3088 DUF4942 domain-containing protein -
MC63_RS16130 (3127400) 3127400..3128404 - 1005 WP_001179707.1 IS110-like element ISSfl8 family transposase -
MC63_RS16135 (3128476) 3128476..3129036 - 561 Protein_3090 DUF4942 domain-containing protein -
MC63_RS16140 (3129121) 3129121..3129318 - 198 WP_000839282.1 DUF957 domain-containing protein -
MC63_RS16145 (3129330) 3129330..3129821 - 492 WP_000976828.1 DUF5983 family protein -
MC63_RS16150 (3129818) 3129818..3130192 - 375 WP_000854917.1 TA system toxin CbtA family protein Toxin
MC63_RS16155 (3130239) 3130239..3130613 - 375 WP_001280958.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MC63_RS16160 (3130776) 3130776..3130997 - 222 WP_001220314.1 DUF987 domain-containing protein -
MC63_RS16165 (3131060) 3131060..3131536 - 477 WP_005011624.1 RadC family protein -
MC63_RS16170 (3131552) 3131552..3132025 - 474 WP_005011597.1 antirestriction protein -
MC63_RS16180 (3132367) 3132367..3133185 - 819 WP_001234651.1 DUF932 domain-containing protein -
MC63_RS16190 (3133303) 3133303..3133498 - 196 Protein_3099 DUF905 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3127400..3128404 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14145.22 Da        Isoelectric Point: 7.7761

>T93305 WP_000854917.1 NZ_CP026098:c3130192-3129818 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T93305 NZ_CP026098:c3130192-3129818 [Shigella flexneri]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13793.62 Da        Isoelectric Point: 6.4651

>AT93305 WP_001280958.1 NZ_CP026098:c3130613-3130239 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT93305 NZ_CP026098:c3130613-3130239 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q93EZ7


Antitoxin

Source ID Structure
AlphaFold DB A0A0K4PAV2

References