Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1210298..1210952 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag MC63_RS06025 Protein ID WP_000244767.1
Coordinates 1210545..1210952 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID Q0T100
Locus tag MC63_RS06020 Protein ID WP_000354044.1
Coordinates 1210298..1210564 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS05995 (1205467) 1205467..1206210 + 744 WP_000951959.1 SDR family oxidoreductase -
MC63_RS06000 (1206267) 1206267..1207700 - 1434 WP_005076748.1 6-phospho-beta-glucosidase BglA -
MC63_RS06005 (1207745) 1207745..1208056 + 312 WP_001182950.1 N(4)-acetylcytidine aminohydrolase -
MC63_RS06010 (1208220) 1208220..1208879 + 660 WP_000250274.1 hemolysin III family protein -
MC63_RS06015 (1209075) 1209075..1210055 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MC63_RS06020 (1210298) 1210298..1210564 + 267 WP_000354044.1 FAD assembly factor SdhE Antitoxin
MC63_RS06025 (1210545) 1210545..1210952 + 408 WP_000244767.1 protein YgfX Toxin
MC63_RS06030 (1210992) 1210992..1211513 - 522 WP_001055867.1 flavodoxin FldB -
MC63_RS06035 (1211625) 1211625..1212521 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MC63_RS06040 (1212546) 1212546..1213256 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MC63_RS06045 (1213262) 1213262..1214995 + 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T93290 WP_000244767.1 NZ_CP026098:1210545-1210952 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T93290 NZ_CP026098:1210545-1210952 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10529.09 Da        Isoelectric Point: 5.1599

>AT93290 WP_000354044.1 NZ_CP026098:1210298-1210564 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT93290 NZ_CP026098:1210298-1210564 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TIU2

References