Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 518381..519012 Replicon chromosome
Accession NZ_CP026098
Organism Shigella flexneri strain FDAARGOS_74

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag MC63_RS02475 Protein ID WP_001259385.1
Coordinates 518381..518656 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MC63_RS02480 Protein ID WP_000593555.1
Coordinates 518653..519012 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC63_RS02460 (513399) 513399..514466 + 1068 WP_000361474.1 HlyD family secretion protein -
MC63_RS02465 (514463) 514463..517198 + 2736 WP_025758458.1 ribosome-associated ATPase/putative transporter RbbA -
MC63_RS02470 (517198) 517198..518322 + 1125 WP_001216257.1 ABC transporter permease -
MC63_RS02475 (518381) 518381..518656 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
MC63_RS02480 (518653) 518653..519012 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MC63_RS02485 (519132) 519132..519533 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MC63_RS02490 (519539) 519539..520345 - 807 WP_000173672.1 nickel import ATP-binding protein NikE -
MC63_RS02495 (520342) 520342..521106 - 765 WP_001136248.1 nickel import ATP-binding protein NikD -
MC63_RS02500 (521106) 521106..521939 - 834 WP_001008949.1 nickel ABC transporter permease subunit NikC -
MC63_RS02505 (521936) 521936..522880 - 945 WP_025758457.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T93288 WP_001259385.1 NZ_CP026098:518381-518656 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T93288 NZ_CP026098:518381-518656 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACACCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT93288 WP_000593555.1 NZ_CP026098:518653-519012 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT93288 NZ_CP026098:518653-519012 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACATTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References