Detailed information of TA system TA093277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 86919..87507
• Replicon: plasmid pOXA58_005069
• Accession: NZ_CP026086
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: C2U64_RS00515
• Protein ID: WP_000438825.1
• Coordinates: 87220..87507 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: C2U64_RS00510
• Protein ID: WP_004728120.1
• Coordinates: 86919..87233 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00465	82855..83127	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C2U64_RS00470	83120..83440	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U64_RS00480	83564..84889	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00490	85068..85487	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C2U64_RS00495	85567..85848	-	282	WP_000985609.1	putative addiction module antidote protein	-
C2U64_RS00500	85849..86151	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U64_RS00505	86300..86830	+	531	WP_000172759.1	hypothetical protein	-
C2U64_RS00510	86919..87233	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
C2U64_RS00515	87220..87507	-	288	WP_000438825.1	BrnT family toxin	Toxin
C2U64_RS00525	87764..89089	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00535	89256..90590	-	1335	WP_103254392.1	ISNCY family transposase	-
C2U64_RS00540	90710..91315	-	606	WP_000447788.1	LysE family translocator	-
C2U64_RS00545	91365..92192	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / mph(E) / msr(E) / blaOXA-58	-	1..112436	112436
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	76888..94913	18025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T93277 WP_000438825.1 NZ_CP026086:c87507-87220 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T93277 NZ_CP026086:c87507-87220 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT93277 WP_004728120.1 NZ_CP026086:c87233-86919 [Acinetobacter pittii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT93277 NZ_CP026086:c87233-86919 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9