Detailed information of TA system TA093276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 85567..86151
• Replicon: plasmid pOXA58_005069
• Accession: NZ_CP026086
• Organism: Acinetobacter pittii strain WCHAP005069

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: C2U64_RS00500
• Protein ID: WP_000286964.1
• Coordinates: 85849..86151 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C2U64_RS00495
• Protein ID: WP_000985609.1
• Coordinates: 85567..85848 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U64_RS00460	80887..82362	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C2U64_RS00465	82855..83127	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C2U64_RS00470	83120..83440	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C2U64_RS00480	83564..84889	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00490	85068..85487	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C2U64_RS00495	85567..85848	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
C2U64_RS00500	85849..86151	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U64_RS00505	86300..86830	+	531	WP_000172759.1	hypothetical protein	-
C2U64_RS00510	86919..87233	-	315	WP_004728120.1	BrnA antitoxin family protein	-
C2U64_RS00515	87220..87507	-	288	WP_000438825.1	BrnT family toxin	-
C2U64_RS00525	87764..89089	-	1326	WP_008942541.1	ISNCY family transposase	-
C2U64_RS00535	89256..90590	-	1335	WP_103254392.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / mph(E) / msr(E) / blaOXA-58	-	1..112436	112436
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	76888..94913	18025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T93276 WP_000286964.1 NZ_CP026086:c86151-85849 [Acinetobacter pittii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T93276 NZ_CP026086:c86151-85849 [Acinetobacter pittii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT93276 WP_000985609.1 NZ_CP026086:c85848-85567 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT93276 NZ_CP026086:c85848-85567 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94