93270

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4137989..4139324 Replicon chromosome
Accession NZ_CP026085
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name sanaT Uniprot ID E0J2C5
Locus tag C1467_RS21470 Protein ID WP_000342500.1
Coordinates 4138377..4139324 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag C1467_RS21465 Protein ID WP_000152741.1
Coordinates 4137989..4138384 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS21435 4133754..4134707 + 954 WP_000290405.1 hypothetical protein -
C1467_RS21440 4134943..4135761 + 819 WP_000761991.1 DUF945 domain-containing protein -
C1467_RS21445 4135791..4136264 + 474 WP_001292742.1 DNA repair protein RadC -
C1467_RS21450 4136264..4136599 + 336 WP_000939438.1 type IV toxin-antitoxin system YeeU family antitoxin -
C1467_RS21455 4136630..4136971 + 342 WP_000691790.1 TA system toxin CbtA family protein -
C1467_RS21460 4137086..4137919 + 834 WP_001192746.1 DUF4942 domain-containing protein -
C1467_RS21465 4137989..4138384 + 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
C1467_RS21470 4138377..4139324 + 948 WP_000342500.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
C1467_RS21475 4139717..4140982 + 1266 WP_001218329.1 integrase arm-type DNA-binding domain-containing protein -
C1467_RS21480 4141183..4142652 - 1470 WP_001022619.1 serine/threonine protein kinase -
C1467_RS21485 4142649..4143062 - 414 WP_001368082.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC / fimD 4120537..4169890 49353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35562.71 Da        Isoelectric Point: 4.9737

>T93270 WP_000342500.1 NZ_CP026085:4138377-4139324 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGSTGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T93270 NZ_CP026085:4138377-4139324 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TGGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGAGCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCTGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGTGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT93270 WP_000152741.1 NZ_CP026085:4137989-4138384 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT93270 NZ_CP026085:4137989-4138384 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGAGCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E0J2C5

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References