Detailed information of TA system TA093269

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4136264..4136971
• Replicon: chromosome
• Accession: NZ_CP026085
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E0J2C8
• Locus tag: C1467_RS21455
• Protein ID: WP_000691790.1
• Coordinates: 4136630..4136971 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C1467_RS21450
• Protein ID: WP_000939438.1
• Coordinates: 4136264..4136599 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1467_RS21425	4132211..4132429	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
C1467_RS21430	4132547..4133161	-	615	WP_000772909.1	inovirus Gp2 family protein	-
C1467_RS21435	4133754..4134707	+	954	WP_000290405.1	hypothetical protein	-
C1467_RS21440	4134943..4135761	+	819	WP_000761991.1	DUF945 domain-containing protein	-
C1467_RS21445	4135791..4136264	+	474	WP_001292742.1	DNA repair protein RadC	-
C1467_RS21450	4136264..4136599	+	336	WP_000939438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C1467_RS21455	4136630..4136971	+	342	WP_000691790.1	TA system toxin CbtA family protein	Toxin
C1467_RS21460	4137086..4137919	+	834	WP_001192746.1	DUF4942 domain-containing protein	-
C1467_RS21465	4137989..4138384	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
C1467_RS21470	4138377..4139324	+	948	WP_000342500.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C1467_RS21475	4139717..4140982	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC / fimD	4120537..4169890	49353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13060.08 Da
• Isoelectric Point: 9.4583

>T93269 WP_000691790.1 NZ_CP026085:4136630-4136971 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T93269 NZ_CP026085:4136630-4136971 [Escherichia coli]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12639.63 Da
• Isoelectric Point: 7.7723

>AT93269 WP_000939438.1 NZ_CP026085:4136264-4136599 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPWQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT93269 NZ_CP026085:4136264-4136599 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGGCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: E0J2C8