Detailed information of TA system TA093265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2483281..2483919
• Replicon: chromosome
• Accession: NZ_CP026085
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C1467_RS12940
• Protein ID: WP_000813794.1
• Coordinates: 2483743..2483919 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C1467_RS12935
• Protein ID: WP_001270285.1
• Coordinates: 2483281..2483697 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1467_RS12915	2478433..2479374	-	942	WP_001251315.1	ABC transporter permease	-
C1467_RS12920	2479375..2480388	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C1467_RS12925	2480406..2481551	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
C1467_RS12930	2481796..2483202	-	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
C1467_RS12935	2483281..2483697	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C1467_RS12940	2483743..2483919	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C1467_RS12945	2484141..2484371	+	231	WP_000494244.1	YncJ family protein	-
C1467_RS12950	2484463..2486424	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C1467_RS12955	2486497..2487033	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C1467_RS12960	2487086..2488300	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2488340..2489488	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T93265 WP_000813794.1 NZ_CP026085:c2483919-2483743 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T93265 NZ_CP026085:c2483919-2483743 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT93265 WP_001270285.1 NZ_CP026085:c2483697-2483281 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT93265 NZ_CP026085:c2483697-2483281 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB