Detailed information of TA system TA093263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2311821..2312347
• Replicon: chromosome
• Accession: NZ_CP026085
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C1467_RS12065
• Protein ID: WP_000323025.1
• Coordinates: 2312060..2312347 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C1467_RS12060
• Protein ID: WP_000534858.1
• Coordinates: 2311821..2312060 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1467_RS12025	2307183..2307539	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
C1467_RS25205	2308150..2308437	+	288	Protein_2210	hypothetical protein	-
C1467_RS12040	2309206..2310519	+	1314	WP_000589005.1	ISNCY family transposase	-
C1467_RS12050	2310956..2311288	-	333	WP_001301033.1	protein FlxA	-
C1467_RS12055	2311491..2311796	-	306	WP_001326990.1	hypothetical protein	-
C1467_RS12060	2311821..2312060	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C1467_RS12065	2312060..2312347	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C1467_RS12070	2312419..2312574	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C1467_RS12075	2312791..2313042	+	252	WP_000980994.1	hypothetical protein	-
C1467_RS12080	2313109..2313387	+	279	WP_012304870.1	hypothetical protein	-
C1467_RS12085	2313389..2314438	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
C1467_RS12090	2314452..2315204	+	753	WP_001047135.1	antitermination protein	-
C1467_RS25000	2315482..2315571	-	90	WP_120795389.1	hypothetical protein	-
C1467_RS12095	2315626..2315838	-	213	WP_000087756.1	cold shock-like protein CspF	-
C1467_RS12100	2316139..2316354	+	216	WP_000066484.1	cold shock-like protein CspB	-
C1467_RS25325	2316718..2316888	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
C1467_RS12105	2317108..2317323	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2286943..2349535	62592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T93263 WP_000323025.1 NZ_CP026085:2312060-2312347 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T93263 NZ_CP026085:2312060-2312347 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT93263 WP_000534858.1 NZ_CP026085:2311821-2312060 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT93263 NZ_CP026085:2311821-2312060 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8