93253

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1329159..1329784 Replicon chromosome
Accession NZ_CP026085
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag C1467_RS06865 Protein ID WP_000911329.1
Coordinates 1329386..1329784 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag C1467_RS06860 Protein ID WP_000450524.1
Coordinates 1329159..1329386 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1467_RS06835 1324961..1325431 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
C1467_RS06840 1325431..1326003 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
C1467_RS06845 1326149..1327027 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
C1467_RS06850 1327044..1328078 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
C1467_RS06855 1328291..1329004 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
C1467_RS06860 1329159..1329386 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
C1467_RS06865 1329386..1329784 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
C1467_RS06870 1329931..1330794 + 864 WP_001267498.1 neutral zinc metallopeptidase -
C1467_RS06875 1330809..1332824 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
C1467_RS06880 1332898..1333596 + 699 WP_000679823.1 esterase -
C1467_RS06885 1333677..1333877 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T93253 WP_000911329.1 NZ_CP026085:1329386-1329784 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T93253 NZ_CP026085:1329386-1329784 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT93253 WP_000450524.1 NZ_CP026085:1329159-1329386 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT93253 NZ_CP026085:1329159-1329386 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References