Detailed information of TA system TA093248

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 49122..49841
• Replicon: chromosome
• Accession: NZ_CP026085
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E0J373
• Locus tag: C1467_RS00280
• Protein ID: WP_001095907.1
• Coordinates: 49122..49442 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E0J374
• Locus tag: C1467_RS00285
• Protein ID: WP_001192122.1
• Coordinates: 49476..49841 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1467_RS00255	44799..45722	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
C1467_RS00260	45833..46909	-	1077	Protein_46	MFS transporter	-
C1467_RS00265	47019..48227	+	1209	WP_006250222.1	IS4-like element ISVsa5 family transposase	-
C1467_RS00280	49122..49442	-	321	WP_001095907.1	TA system toxin CbtA family protein	Toxin
C1467_RS00285	49476..49841	-	366	WP_001192122.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C1467_RS00290	49873..50094	-	222	WP_000691985.1	DUF987 family protein	-
C1467_RS00295	50103..50573	-	471	WP_000104319.1	DNA repair protein RadC	-
C1467_RS00300	50643..51464	-	822	WP_000197395.1	DUF945 domain-containing protein	-
C1467_RS00305	51536..52012	-	477	WP_001254693.1	hypothetical protein	-
C1467_RS00315	52229..53032	-	804	WP_001245321.1	hypothetical protein	-
C1467_RS00320	53264..54426	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
C1467_RS00325	54457..54747	-	291	Protein_56	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	47019..48227	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12184.76 Da
• Isoelectric Point: 5.5740

>T93248 WP_001095907.1 NZ_CP026085:c49442-49122 [Escherichia coli]
MNTPTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVRRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T93248 NZ_CP026085:c49442-49122 [Escherichia coli]
ATGAATACTCCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTTCTTGTGGAACGATTCGAGCTGGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCCTGGATAACATCTTTGGATGTTCGTCGTGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13470.22 Da
• Isoelectric Point: 6.8421

>AT93248 WP_001192122.1 NZ_CP026085:c49841-49476 [Escherichia coli]
MQSTTLSGTSAENVSCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT93248 NZ_CP026085:c49841-49476 [Escherichia coli]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGTATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
GTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Toxin

• AlphaFold DB: E0J373

Antitoxin

• AlphaFold DB: E0J374