Detailed information of TA system TA093243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2690149..2690678
• Replicon: chromosome
• Accession: NZ_CP026080
• Organism: Staphylococcus aureus strain FDAARGOS_12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK70_RS13850
• Protein ID: WP_000621175.1
• Coordinates: 2690316..2690678 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK70_RS13845
• Protein ID: WP_000948331.1
• Coordinates: 2690149..2690319 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK70_RS13815	2685185..2685745	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
RK70_RS13820	2685954..2686433	+	480	WP_001287088.1	hypothetical protein	-
RK70_RS13825	2686426..2688009	+	1584	WP_001294626.1	PH domain-containing protein	-
RK70_RS13830	2687996..2688487	+	492	WP_001205910.1	PH domain-containing protein	-
RK70_RS13835	2688491..2688850	+	360	WP_000581200.1	holo-ACP synthase	-
RK70_RS13840	2688916..2690064	+	1149	WP_001281145.1	alanine racemase	-
RK70_RS13845	2690149..2690319	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK70_RS13850	2690316..2690678	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK70_RS13860	2691027..2692017	+	991	Protein_2561	PP2C family protein-serine/threonine phosphatase	-
RK70_RS13865	2692136..2692462	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RK70_RS13870	2692464..2692943	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK70_RS13875	2692918..2693688	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T93243 WP_000621175.1 NZ_CP026080:2690316-2690678 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T93243 NZ_CP026080:2690316-2690678 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT93243 WP_000948331.1 NZ_CP026080:2690149-2690319 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT93243 NZ_CP026080:2690149-2690319 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1