Detailed information of TA system TA093222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1566518..1567047
• Replicon: chromosome
• Accession: NZ_CP026079
• Organism: Staphylococcus aureus strain FDAARGOS_9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RK67_RS08000
• Protein ID: WP_000621175.1
• Coordinates: 1566685..1567047 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: RK67_RS07995
• Protein ID: WP_000948331.1
• Coordinates: 1566518..1566688 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RK67_RS07965	1561555..1562115	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
RK67_RS07970	1562323..1562802	+	480	WP_001287087.1	hypothetical protein	-
RK67_RS07975	1562795..1564378	+	1584	WP_001294626.1	PH domain-containing protein	-
RK67_RS07980	1564365..1564856	+	492	WP_001205912.1	PH domain-containing protein	-
RK67_RS07985	1564860..1565219	+	360	WP_000581197.1	holo-ACP synthase	-
RK67_RS07990	1565285..1566433	+	1149	WP_001281154.1	alanine racemase	-
RK67_RS07995	1566518..1566688	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
RK67_RS08000	1566685..1567047	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RK67_RS08010	1567397..1568398	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
RK67_RS08015	1568517..1568843	+	327	WP_001052491.1	anti-sigma factor antagonist	-
RK67_RS08020	1568845..1569324	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
RK67_RS08025	1569299..1570069	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T93222 WP_000621175.1 NZ_CP026079:1566685-1567047 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T93222 NZ_CP026079:1566685-1567047 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT93222 WP_000948331.1 NZ_CP026079:1566518-1566688 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT93222 NZ_CP026079:1566518-1566688 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1